Research Article

Elucidation of microbial decolourization and degradation efficiency of recalcitrant-carcinogenic azo dye congo red by novel bacterial strains

Shinkafi M. S.1*, GALADIMA M.S2, Mohammed I. U.3, Hassan A. S.3, J. M. Hayatu4
1 Department of Pure and Industrial Chemistry, College of Nature and Pharmaceutical Science, Bayero University Kano
2 Department of Water Resource and Environmental Engineering Ahmadu Bello University Zaria
3 Department of Research and Technical Services, National Water Resources Institute, Kaduna, Nigeria
4 Department of Training, National Water Resources Institute, Kaduna, Nigeria
* Corresponding author: ibrahimum222@yahoo.com
Published: Jun, 2015
Pages: 114-123

Abstract

Novel bacterial strains capable of decolorizing textile dyes were isolated from dye contaminated soil obtained from the vicinity of African Textiles Industry Challawa, Kano - Nigeria. The Carcinogenic azo dye Congo red used widely in various industrial processes poses terrific environmental concern, causing major health problems to human beings. In the present study, decolourization of Congo red by newly isolated antibiotics sensitive novel bacterial strains from dyes contaminated soil of a treatment plant capable of decolorizing organic dyes were investigated and identified as Rhodococcus rhodochrous and Pseudomonas aeruginosa strains by 16S rDNA gene sequence analysis. Decolorization of Congo red dye using these bacterial strains was assayed by screening and optimizing various parameters to determine the optimal conditions required for maximum decolourization. Rhodococcus rhodochrous and Pseudomonas aeruginosa showed maximum decolourization upon incubation for 24 hrs at 37 °C and pH 7. Enhanced decolourization was achieved by amendment with glucose and peptone as well as carbon and nitrogen sources in the culture medium. The bacterial strains showed great potential in the decolourization of Congo red dye up to 96.1 % for Rhodococcus rhodocchrous and 91.1 % for Pseudomonas aeruginosa in the aqueous environment at optimal conditions. High performance liquid chromatography (HPLC) analysis confirmed that dye decolourization occurred due to the breakdown of dye molecules into colourless end products. The less toxic nature of the dye degraded products was observed by microbial toxicity assay of agricultural significant bacteria and this can be exploited for the bioremediation of different classes of textile dyes and their derivatives containing waste

Keywords

Antibiotics sensitive bacterial strains decolourization microbial toxicity textile dyes

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How to Cite

S., S. M., M.S, G., U., M. I., S., H. A., & Hayatu, J. M. . (2015). Elucidation of microbial decolourization and degradation efficiency of recalcitrant-carcinogenic azo dye congo red by novel bacterial strains. Nigerian Journal of Materials Science and Engineering, 6(1), 114-123.

S. M. S., G. M.S, M. I. U., H. A. S., and J. M. . Hayatu, "Elucidation of microbial decolourization and degradation efficiency of recalcitrant-carcinogenic azo dye congo red by novel bacterial strains," Nigerian Journal of Materials Science and Engineering, vol. 6, no. 1, pp. 114-123, June 2015.

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